The Role of Hypoxia-Inducible Factor 1α in Determining the Properties of Castrate-Resistant Prostate Cancers

WKB Ranasinghe; L Xiao; S Kovac; M Chang; C Michiels; D Bolton; A Shulkes; GS Baldwin; O Patel

Journal article

The Role of Hypoxia-Inducible Factor 1α in Determining the Properties of Castrate-Resistant Prostate Cancers

WKB Ranasinghe, L Xiao, S Kovac, M Chang, C Michiels, D Bolton, A Shulkes, GS Baldwin, O Patel

Plos One | PUBLIC LIBRARY SCIENCE | Published : 2013

DOI: 10.1371/journal.pone.0054251

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Abstract

Background: Castrate-resistant prostate cancer (CRPC) is a lethal condition in patients receiving androgen deprivation therapy for prostate cancer (PC). Despite numerous studies showing the expression of HIF1α protein under normoxia in PC cell lines, the role of this normoxic HIF1α expression in chemo-resistance and migration has not been investigated previously. As no method is currently available to determine which tumors will progress to CRPC, the role of HIF1α in PC and its potential for predicting the development of CRPC was also investigated. Methods: The effect of HIF1α protein knockdown on chemo-resistance and migration of PC3 cells was assessed by cell counting and Transwell assays,..

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University of Melbourne Researchers

Damien Bolton Author

Arthur Shulkes Author

Oneel Patel Author Surgery - Austin Health

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Grants

Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

This work was supported by grants 454322 (GSB), 566555 (GSB, AS) and 628390 (AS, OP, GSB) from the National Health and Medical Research Council of Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.